Paper ID: 109
1 The Novo Nordisk Foundation Center for Biosustainability Technical University of Denmark (Denmark)
2 Department of Chemical Engineering Lund University (Sweden)
Besides its role as an important model for the study of fundamental cellular processes in eukaryotes, the yeast Saccharomyces cerevisiae is also the organism of choice for many biotechnological applications. Due to its excellent performance in established large-scale processes and well-studied genetics, S. cerevisiae can be modified to host processes leading to conversion of biomass to valuable chemicals as a sustainable alternative to the production of such chemicals from oil. Economic viability of the biorefinery concept of using biomass instead of fossil resources for production of fuels and chemicals needs the development of industrial strains that are sufficiently robust, utilize low value waste biomass streams rich in C5 sugars that are not naturally fermented by S. cerevisiae, and produce added-value chemicals. Here we demonstrate construction of such a yeast cell factory. We present the entire process from the development of advanced molecular tools for engineering of industrial yeast strains, adaptive evolution approaches for generation of platform strains with advanced xylose utilization properties and tolerance to a lignocellulosic biomass feedstock, to construction of strains engineered for production of dicarboxylic acids. These can serve as monomers for development of novel biodegradable polymers. Next generation sequencing analysis of the platform strains performing in xylose-rich media and identification of causative mutations will be presented. Furthermore, relevant metabolic engineering strategies and strain characterization will be shown in detail. This project is part of BioREFINE-2G (www.biorefine2g.eu), which is co-funded by the European Commission in the 7th Framework Programme (Project No. FP7-613771).