Paper ID: 253
Chalmers University of Technology (Sweden)
Metabolism represents the core of cellular functions and all cellular processes interact with metabolism through the use of specific metabolites, free energy and/or electron flows. However, metabolism is highly complex involving a large number of chemical reactions, and it is therefore difficult to study metabolism. The many different reactions are traditionally grouped into pathways with dedicated functions, but recent analysis of metabolism has shown that there is a high degree of connectivity between these pathways due to common sharing of co-factors and key metabolites. Also regulation of metabolism is complex due to the requirements for maintaining cellular homeostasis. In this talk I will give illustrations of how different parts of cellular metabolism are connected, i.e. central carbon metabolism, lipid metabolism and protein secretion. I will illustrate how metabolism can be modelled at the genome-scale and how incorporation of protein crowding may be a key determinant for cellular function, and keep proteome homeostasis is an important driver for maintaining proper cellular function. Finally, I will discuss how regulation of metabolism can be studied using different omics analysis, e.g. how we can get new insight into transcriptional regulation through ChIP-exo analysis.