YEAST 2017

28th International Conference on Yeast Genetics and Molecular Biology (ICYGMB)

August 27 – September 1, 2017
Prague, Czech Republic

Paper ID: 345

Investigating role of phosphorylation of the RNA polymerase II in transcription and 3’end formation of functional mRNA

Kecman Tea, Kus Krzysztof, Mohammed Shabaz, Vasiljeva Lidia

University of Oxford, Biochemistry Department, Oxford (United Kingdom)


Reversible phosphorylation of the C-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) is important for coordinated regulation of transcription and maturation of functional mRNA, yet the molecular mechanism underpinning CTD function remains unclear. 

CTD consists of multiple repeats of the consensus sequence (Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7) where it can be phosphorylated on Y1, S2, T4, S5, and S7P. While regulation and function of S2P have been extensively studied, role of T4P as well as kinases and phosphatases responsible for regulation of this recently identified modification are not known. We observe that S2P, T4P and Y1P co-occur on CTD at the end of the transcription cycle in fission yeast suggesting that these marks might be important at this stage. We show that conserved PP1 phosphatase is involved in T4P regulation and lack of its activity leads to genome –wide transcription termination defect. Our findings reinforce the importance of CTD phosphorylation in regulation of Pol II transcription and provide mechanistic insights into CTD function.

RNA polymerase II, Reversible phosphorylation, Pol II transcription
Presented as:
  Oral presentation [S2-1] in S2 RNA processing and regulation

Institute of Microbiology

YEAST 2017
28th International Conference on Yeast Genetics and Molecular Biology (ICYGMB)

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